In vivo demonstration of acid phosphatase activity in the rhizosphere of soil-grown plants

Abstract

For in vivo demonstration of acid phosphatase activity in the rhizosphere of soil-grown plants filter papers were treated with a mixture of 1-naphthyl phosphate as substrate and the diazonium salt Fast Red TR as an indicator. After enzymatic hydrolysis, 1-naphthol forms a red complex with Fast Red TR. This method was applied to 8-day old maize plants and 3… (More)
DOI: 10.1007/BF00012876

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