In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta.

@article{Pavlov2001InVC,
  title={In vivo consequences of putative active site mutations in yeast DNA polymerases alpha, epsilon, delta, and zeta.},
  author={Youri I. Pavlov and Polina V. Shcherbakova and T. A. Kunkel},
  journal={Genetics},
  year={2001},
  volume={159 1},
  pages={47-64}
}
Several amino acids in the active site of family A DNA polymerases contribute to accurate DNA synthesis. For two of these residues, family B DNA polymerases have conserved tyrosine residues in regions II and III that are suggested to have similar functions. Here we replaced each tyrosine with alanine in the catalytic subunits of yeast DNA polymerases alpha, delta, epsilon, and zeta and examined the consequences in vivo. Strains with the tyrosine substitution in the conserved SL/MYPS/N motif in… CONTINUE READING

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