In vivo characterization of regulatory polymorphisms by allele-specific quantification of RNA polymerase loading

@article{Knight2003InVC,
  title={In vivo characterization of regulatory polymorphisms by allele-specific quantification of RNA polymerase loading},
  author={Julian C. Knight and Brendan J. Keating and Kirk A. Rockett and Dominic P Kwiatkowski},
  journal={Nature Genetics},
  year={2003},
  volume={33},
  pages={469-475}
}
In vivo characterization of regulatory polymorphisms is a key requirement for next-generation human genetic analysis. Here we describe haploChIP, a method that uses chromatin immunoprecipitation (ChIP) and mass spectrometry to identify differential protein–DNA binding in vivo associated with allelic variants of a gene. We demonstrate this approach with the imprinted gene SNRPN. HaploChIP showed close correlation between the level of bound phosphorylated RNA polymerase II at the SNRPN locus and… CONTINUE READING