In vivo chaperone activity of heat shock protein 70 and thermotolerance.

  title={In vivo chaperone activity of heat shock protein 70 and thermotolerance.},
  author={Ellen A. A. Nollen and Jeanette F. Brunsting and H. W. Roelofsen and L Arthur Weber and Harm H. Kampinga},
  journal={Molecular and cellular biology},
  volume={19 3},
Heat shock protein 70 (Hsp70) is thought to play a critical role in the thermotolerance of mammalian cells, presumably due to its chaperone activity. We examined the chaperone activity and cellular heat resistance of a clonal cell line in which overexpression of Hsp70 was transiently induced by means of the tetracycline-regulated gene expression system. This single-cell-line approach circumvents problems associated with clonal variation and indirect effects resulting from constitutive… CONTINUE READING


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