In vivo bioluminescence imaging for the study of intestinal colonization by Escherichia coli in mice.

@article{Foucault2010InVB,
  title={In vivo bioluminescence imaging for the study of intestinal colonization by Escherichia coli in mice.},
  author={M. Foucault and Lee J. Thomas and Sylvie Goussard and Bruce R. Branchini and Catherine Grillot-Courvalin},
  journal={Applied and environmental microbiology},
  year={2010},
  volume={76 1},
  pages={264-74}
}
Bioluminescence imaging (BLI) is emerging as a powerful tool for real-time monitoring of infections in living animals. However, since luciferases are oxygenases, it has been suggested that the requirement for oxygen may limit the use of BLI in anaerobic environments, such as the lumen of the gut. Strains of Escherichia coli harboring the genes for either the bacterial luciferase from Photorhabdus luminescens or the PpyRE-TS and PpyGR-TS firefly luciferase mutants of Photinus pyralis (red and… CONTINUE READING
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