In vivo augmentation of IFN-gamma with a rIL-12 human/mouse chimera: pleiotropic effects against infectious agents in mice and rats.

@article{Gladue1994InVA,
  title={In vivo augmentation of IFN-gamma with a rIL-12 human/mouse chimera: pleiotropic effects against infectious agents in mice and rats.},
  author={Ronald P. Gladue and A M Laquerre and H A Magna and Lisa A. Carroll and Marie O'Donnell and Paul S. Changelian and Arthur E. Franke},
  journal={Cytokine},
  year={1994},
  volume={6 3},
  pages={318-28}
}
A heterodimer containing the mouse 35 kDa and human 40 kDa subunit of IL-12 was expressed in COS cells (cIL-12). Administration of 25-200 U of the cIL-12-COS supernatant to mice twice daily for 2 days augmented spleen cell IFN-gamma production in response to IL-2 and peritoneal macrophage activity (superoxide and nitrites) as compared to animals receiving mock transfected supernatants. cIL-12 also increased levels of IFN-gamma in serum but most dramatically following an LPS injection (50-fold… CONTINUE READING