In vivo and in vitro regulation of erythropoietin mRNA: measurement by competitive polymerase chain reaction.

@article{Fandrey1993InVA,
  title={In vivo and in vitro regulation of erythropoietin mRNA: measurement by competitive polymerase chain reaction.},
  author={Joachim Fandrey and H. Franklin Bunn},
  journal={Blood},
  year={1993},
  volume={81 3},
  pages={617-23}
}
The regulation of erythropoietin (Epo) production was investigated by competitive polymerase chain reaction, a highly sensitive and accurate means of measuring Epo mRNA levels. Co-amplification of the test sample with added mutant Epo cDNA template corrects for variability in the efficiency of amplification. Epo mRNA levels were determined in tissues of normal rats and in animals with varying degrees of anemia. Reduction of the hematocrit level from 0.40 to 0.15-0.20 resulted in a 300-fold… CONTINUE READING