• Medicine
  • Published in Journal of molecular biology 1999

In vivo and in vitro activities of the Escherichia coli sigma54 transcription activator, PspF, and its DNA-binding mutant, PspFDeltaHTH.

@article{Jovanovic1999InVA,
  title={In vivo and in vitro activities of the Escherichia coli sigma54 transcription activator, PspF, and its DNA-binding mutant, PspFDeltaHTH.},
  author={Goran Jovanovic and Jasna Rakonjac and Peter Model},
  journal={Journal of molecular biology},
  year={1999},
  volume={285 2},
  pages={
          469-83
        }
}
Transcription of the phage-shock protein (psp) operon in Escherichia coli is driven by a sigma54 promoter, stimulated by integration host factor and dependent on an upstream, cis-acting sequence and an activator protein, PspF. PspF belongs to the enhancer binding protein family but lacks an N-terminal regulatory domain. Purified PspF is not modified and has an ATPase activity that is increased twofold in the presence of DNA carrying the psp cis-acting sequence. Purified mutant His-tagged PspF… CONTINUE READING

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