In vivo DNA-binding properties of a yeast transcription activator protein.

@article{Selleck1987InVD,
  title={In vivo DNA-binding properties of a yeast transcription activator protein.},
  author={Scott B. Selleck and John E. Majors},
  journal={Molecular and cellular biology},
  year={1987},
  volume={7 9},
  pages={
          3260-7
        }
}
UV light can serve as a molecular probe to identify DNA-protein interactions at nucleotide level resolution from intact yeast cells. We have used the photofootprinting technique to determine during which of three regulated states (uninduced, induced, and catabolite repressed) the transcriptional activator protein encoded by GAL4 binds to its recognition sites within the GAL1-GAL10 upstream activating sequence (UASG). GAL4 protein is bound to at least four, and probably five, related sequence… CONTINUE READING

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