In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.

@article{Yamane1987InVT,
  title={In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.},
  author={Kazuhiko Yamane and Shigeyasu Ichihara and Shoji Mizushima},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 5},
  pages={2358-62}
}
The energy requirement for protein translocation across membrane was studied with inverted membrane vesicles from an Escherichia coli strain that lacks all components of F1F0-ATPase. An ompF-lpp chimeric protein was used as a model secretory protein. Translocation of the chimeric protein into membrane vesicles was totally inhibited in the presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP) or valinomycin and nigericin and partially inhibited when either valinomycin or nigericin alone… CONTINUE READING