In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV).

@article{Boga1992InVT,
  title={In vitro translation of a subgenomic mRNA from purified virions of the Spanish field isolate AST/89 of rabbit hemorrhagic disease virus (RHDV).},
  author={Jos{\'e} Antonio Boga and M Soledad Mar{\'i}n and R C B Casais and Miguel Prieto and Francisco Vargas Parra},
  journal={Virus research},
  year={1992},
  volume={26 1},
  pages={33-40}
}
Purified preparations of the Spanish field isolate of rabbit hemorrhagic disease virus AST/89 were found to contain the plus-stranded genomic RNA of more than 7.4 kilobases (kb) and large amounts of a subgenomic mRNA of 2.4 kb. The smaller RNA was translated in vitro and shown to code for a 60 kDa protein which was immunoprecipitated using anti-RHDV as well as anti-VP60 sera.