In vitro transfer of Ga-67 from transferrin to ferritin.

  title={In vitro transfer of Ga-67 from transferrin to ferritin.},
  author={Ronald E. Weiner and George J. Schreiber and Paul B. Hoffer},
  journal={Journal of nuclear medicine : official publication, Society of Nuclear Medicine},
  volume={24 7},
Equilibrium dialysis was used to examine the binding of Ga-67 to horse spleen ferritin (HFE), and the ability of this protein to remove Ga-67 originally bound to human transferrin (TF). Seventy hours were required for the HFE to bind 70% of the activity. When HFE was placed in competition with preformed TF-Ga-67 complex, little nuclide was translocated to HFE. Upon the addition of compounds of low molecular weight that occur intracellularly, this transfer was dramatically enhanced. In the… CONTINUE READING