In vitro transcription initiation of the spinach chloroplast 16S rRNA gene at two tandem promoters.

@article{Lescure1985InVT,
  title={In vitro transcription initiation of the spinach chloroplast 16S rRNA gene at two tandem promoters.},
  author={A M Lescure and Cordelia Bisanz-Seyer and H{\'e}l{\`e}ne Pesey and R. Mache},
  journal={Nucleic acids research},
  year={1985},
  volume={13 24},
  pages={8787-96}
}
Two potential prokaryotic promoters, P1 and P2, are characterized 164 and 114 bp upstream of the spinach chloroplast 16S rRNA gene. The strengths of these promoters, calculated according to an homology score established for E. coli RNA-polymerase, are identical. Experiments performed with a Taq I-DNA fragment, containing 16 bp of the 16S rDNA and 243 bp upstream of the gene, give evidence that in vitro, E. coli RNA-polymerase starts transcription at these two promoters. These results are based… CONTINUE READING