In vitro transcription catalyzed by heat-treated human rotavirus.

@article{Spencer1981InVT,
  title={In vitro transcription catalyzed by heat-treated human rotavirus.},
  author={Elizabeth M. Spencer and M. L. D{\'a}vila Arias},
  journal={Journal of virology},
  year={1981},
  volume={40 1},
  pages={
          1-10
        }
}
The in vitro characteristics of human rotavirus transcription have been examined. The virus has an associated RNA polymerase activity which was activated after a heat shock treatment. The enzyme required the presence of the four ribonucleoside triphosphates and a divalent cation (Mg2+), and it required an optimum pH of 8.5. The polymerase was activated by monovalent salts and inhibited by Na PPi. The addition of actinomycin D, alpha-amanitin, or rifampin did not inhibit the polymerase activity… CONTINUE READING

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