In vitro transcription by wheat germ RNA polymerase II. Initiation of RNA synthesis on relaxed, closed circular template.

Abstract

We have developed a novel method for studying the ability to wheat germ RNA polymerase II to initiate transcription in vitro on unnicked duplex DNA template. Our template is a plasmid which has been relaxed in vitro with wheat germ DNA topoisomerase I. The plasmid consists of SV40 cloned in pBR322. We transcribe briefly to synthesize highly labeled nascent… (More)

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Cite this paper

@article{Dynan1981InVT, title={In vitro transcription by wheat germ RNA polymerase II. Initiation of RNA synthesis on relaxed, closed circular template.}, author={Wffliam S Dynan and Robert Burgess}, journal={The Journal of biological chemistry}, year={1981}, volume={256 11}, pages={5866-73} }