In vitro template activity of 0.3 mRNA from wild type and initiation mutants of bacteriophage T7

Abstract

Bacteriophage T7 0.3 mRNA synthesised and processed in vitro has been purified starting from the DNA of T7+ as well as from that of two initiation mutants of T7 (CR17 with a U → C transition in the initiation codon and CR35b whose potential Shine and Dalgarno (S-D) interaction is interrupted by a G → A transition). These mRNAs were used as templates to… (More)
DOI: 10.1007/BF00334091

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