In vitro synthesis of rat brain hexokinase.


Hexokinase (ATP:D-hexose 6-phosphotransferase, EC has been synthesized in the rabbit reticulocyte lysate system directed by poly(A)+ mRNA isolated from rat brain. Identification of the in vitro synthesis product as hexokinase was based on its immunoprecipitation with anti-hexokinase serum as well as the generation of identical peptide maps after… (More)


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