In vitro synthesis of native myosin, actin, and tropomyosin from embryonic chick polyribosomes.

@article{Heysood1968InVS,
  title={In vitro synthesis of native myosin, actin, and tropomyosin from embryonic chick polyribosomes.},
  author={S M Heysood and A. Rich},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1968},
  volume={59 2},
  pages={590-7}
}
There is a low level of free ribonuclease present in embryonic chick muscle, and the ribosomes are not attached to an endoplasmic reticulum. Thus, it is easy to isolate the polyribosomes (polysomes) in a relatively undegraded fashion. In a previous study we have shown that it is possible to use the undegraded polysomes isolated from embryonic chick leg… CONTINUE READING