In vitro synthesis of heparosan using recombinant Pasteurella multocida heparosan synthase PmHS2

@inproceedings{Chavaroche2009InVS,
  title={In vitro synthesis of heparosan using recombinant Pasteurella multocida heparosan synthase PmHS2},
  author={Ana{\"i}s A. E. Chavaroche and Jan Springer and Floor K. Kooy and Carmen G Boeriu and Gerrit Eggink},
  booktitle={Applied Microbiology and Biotechnology},
  year={2009}
}
In vertebrates and bacteria, heparosan the precursor of heparin is synthesized by glycosyltransferases via the stepwise addition of UDP-N-acetylglucosamine and UDP-glucuronic acid. As heparin-like molecules represent a great interest in the pharmaceutical area, the cryptic Pasteurella multocida heparosan synthase PmHS2 found to catalyze heparosan synthesis using substrate analogs has been studied. In this paper, we report an efficient way to purify PmHS2 and to maintain its activity stable… CONTINUE READING
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