In vitro study of nuclear assembly and nuclear import using Xenopus egg extracts.

@article{Chan2006InVS,
  title={In vitro study of nuclear assembly and nuclear import using Xenopus egg extracts.},
  author={Rene C. Chan and Douglass I Forbes},
  journal={Methods in molecular biology},
  year={2006},
  volume={322},
  pages={289-300}
}
Nuclear import is a critical process for the cell: molecules are selectively permitted into the nuclear interior where the sheltered genome resides. The process of nuclear import can be biochemically studied in vitro using nuclei reconstituted from Xenopus egg extract components and Xenopus sperm chromatin. This in vitro system allows for the visualization of nuclear import by monitoring the accumulation of fluorescent nuclear import substrates in the reconstituted nuclei. A powerful aspect of… CONTINUE READING