In vitro selection of sialic acid specific RNA aptamer and its application to the rapid sensing of sialic acid modified sugars.

@article{Cho2013InVS,
  title={In vitro selection of sialic acid specific RNA aptamer and its application to the rapid sensing of sialic acid modified sugars.},
  author={Suhyung Cho and Byung Kook Lee and Byung-Kwan Cho and Jeewoong Kim and Byung-Gee Kim},
  journal={Biotechnology and bioengineering},
  year={2013},
  volume={110 3},
  pages={
          905-13
        }
}
Sialic acids (SAs) are located on the terminal positions of glycan on a cell surface, which play important role in the spread and metastasis of cancer cells and infection of pathogen. For their detection and diagnosis, the finding of SA specific ligand is an essential prerequisite. Here, RNA aptamer for N-acetylneuraminic acid (Neu5Ac), a representative of SAs, with the high affinity of 1.35 nM and the selectivity was screened by in vitro selection method. The strong binding of the screened… CONTINUE READING
BETA