In vitro selection of NaCl-tolerant callus lines and regeneration of plantlets in a bamboo (Dendrocalamus strictus Nees)

@article{Singh2003InVS,
  title={In vitro selection of NaCl-tolerant callus lines and regeneration of plantlets in a bamboo (Dendrocalamus strictus Nees)},
  author={Madhulika Singh and Uma Jaiswal and Vijai Shanker Jaiswal},
  journal={In Vitro Cellular & Developmental Biology - Plant},
  year={2003},
  volume={39},
  pages={229-233}
}
Sodium chloride-tolerant plantlets of Dendrocalamus strictus were regenerated successfully from NaCl-tolerant embryogenic callus via somatic embryogenesis. The selection of embryogenic callus tolerant to 100 mM NaCl was made by exposing the callus to increasing (0–200 mM) concentrations of NaCl in Murashige and Skoog medium having 3% (w/v) sucrose, 0.8% (w/v) agar, 3.0 mg l−1 (13.6 μM) 2,4-dichlorophenoxyacetic acid (2,4-D), and 0.5mg l−1 (2.3μM) kinetin (callus initiation medium). The… CONTINUE READING

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