In vitro selection of DNAs with an increased propensity to form small circles.

@article{Rosanio2015InVS,
  title={In vitro selection of DNAs with an increased propensity to form small circles.},
  author={Gabriel Rosanio and Jonathan Widom and O. C. Uhlenbeck},
  journal={Biopolymers},
  year={2015},
  volume={103 6},
  pages={303-20}
}
A protocol was devised to select for DNA molecules that efficiently form circles from a library of 126 base pair DNAs containing 90 randomized base pairs. After six rounds of selection, individual molecules from the library showed 20- to 100-fold greater j-factors compared with the starting library, validating the selection protocol. High-throughput… CONTINUE READING