In vitro regulation of rat Sertoli cell inhibin messenger RNA levels by transforming growth factor-beta 1 and tumour necrosis factor alpha.

Abstract

In the present study, we examined the in vitro regulation of 20-day-old rat Sertoli cell inhibin alpha- and beta B-subunits mRNA levels by transforming growth factor-beta 1 (TGF-beta 1) and tumour necrosis factor alpha (TNF alpha), two factors produced in the testis. Addition of TGF-beta 1 to highly purified cultured Sertoli cells resulted in a time- and dose-dependent enhancement in the alpha-subunit mRNA levels (ED50 = 2.4 pM; maximal increase of 2.6-fold after 48 h of treatment), without affecting the beta B-subunit mRNA levels. Similarly, activin A up-regulated the alpha- but did not modulate the beta B-subunit mRNA levels. By contrast, TNF alpha decreased in a time- and dose-dependent fashion the mRNA levels of the two inhibin subunits alpha and beta B (IC50 = 29 pM for both subunits; maximal decrease of 4.4- and of 4-fold after 72 and 24 h of treatment for respectively the alpha- and beta B-subunits). The effects of TGF-beta 1 and TNF alpha on inhibin mRNA levels occurred within a dose range that might be expected under physiological conditions. In addition, TGF-beta 1-treated Sertoli cells responded to FSH or dibutyryl cyclic AMP ((Bu)2cAMP) by a further and significant additive increase of the alpha-subunit mRNA levels. TNF alpha-treated Sertoli cells responded significantly to FSH and to (Bu)2cAMP, thus attenuating the inhibitory action of TNF alpha on the alpha-inhibin mRNA levels. Together, the present findings emphasize the ability of some local growth factors to modulate the effects of FSH on Sertoli cell function.

Statistics

0100200300'98'00'02'04'06'08'10'12'14'16
Citations per Year

261 Citations

Semantic Scholar estimates that this publication has 261 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{MagueresseBattistoni1995InVR, title={In vitro regulation of rat Sertoli cell inhibin messenger RNA levels by transforming growth factor-beta 1 and tumour necrosis factor alpha.}, author={Brigitte le Magueresse-Battistoni and Anne - Marie Morera and Mohamed Benahmed}, journal={The Journal of endocrinology}, year={1995}, volume={146 3}, pages={501-8} }