In vitro regeneration of Vigna unguiculata (L.) Walp. cv. Blackeye cowpea via shoot organogenesis

@article{Mao2006InVR,
  title={In vitro regeneration of Vigna unguiculata (L.) Walp. cv. Blackeye cowpea via shoot organogenesis},
  author={Jing Mao and Mohsin Abbas Zaidi and John T Arnason and Illimar Altosaar},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2006},
  volume={87},
  pages={121-125}
}
An in vitro regeneration system was developed in cowpea [Vigna unguiculata (L.) Walp.] Blackeye. Among several explants studied, shoot initiation response was observed from shoot apices of 3–5-day-old seedlings. The optimal medium for maximum shoot initiation comprised MS salts, B5 vitamins, 8.88 µM N6-benzylaminopurine, 1 gl-1 casein hydrolysate, 342 μM L-glutamine, 3% sucrose, 0.3% phytagel, adjusted to pH 5.8. A shift in pH from 5.8 to 7.0 had no effect on shoot initiation and on number of… CONTINUE READING

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A review of regeneration and genetic transformation in cowpea (Vigna unguiculata L. Walp)

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