In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants

@article{Feyissa2005InVR,
  title={In vitro regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. (Rosaceae) from leaf explants},
  author={Tileye Feyissa and Margareta Welander and Legesse Negash},
  journal={Plant Cell Reports},
  year={2005},
  volume={24},
  pages={392-400}
}
A protocol for shoot regeneration of Hagenia abyssinica (Bruce) J.F. Gmel. has been developed using leaf explants originating from in vitro seedlings and mature material. The explants were cultured on Murashige and Skoog medium containing various concentrations of α-naphthaleneacetic acid and thidiazuron (TDZ). Concentrations of TDZ lower than 1.0 μM promoted direct shoot regeneration, but higher concentrations promoted callus induction. Around 96–100% regeneration was obtained between 1.0 and… CONTINUE READING
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