In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.

@article{Arnaud1996InVR,
  title={In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis.},
  author={Mary Arnaud and Michel D{\'e}barbouill{\'e} and G. N. Rapoport and Milton H. Saier and Jonathan Reizer},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 31},
  pages={18966-72}
}
Expression of the sacPA and sacB genes of Bacillus subtilis is positively modulated by transcriptional regulatory proteins encoded by the sacT and sacY genes, respectively. Previous genetic studies led to the suggestion that SacT and SacY function as nascent mRNA binding proteins preventing early termination of transcription at terminators located in the leader regions of the corresponding genes. Here we report the overproduction, purification to near homogeneity, and characterization of the… CONTINUE READING