Corpus ID: 235731489

In vitro reconstitution of microtubule dynamics and severing imaged by label-free interference reflection microscopy

  title={In vitro reconstitution of microtubule dynamics and severing imaged by label-free interference reflection microscopy},
  author={Yin-wei Kuo and Jonathon Howard},
The dynamic architecture of the microtubule cytoskeleton is crucial for cell division, motility and morphogenesis. The dynamic properties of microtubules - growth, shrinkage, nucleation and severing - are regulated by an arsenal of microtubule-associated proteins (MAPs). The activities of many of these MAPs have been reconstituted in vitro using microscope assays. As an alternative to fluorescence microscopy, interference-reflection microscopy (IRM) has been introduced as an easy-to-use, wide… Expand

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