In vitro reconstitution of cdc42-mediated actin assembly using purified components.

@article{Ho2006InVR,
  title={In vitro reconstitution of cdc42-mediated actin assembly using purified components.},
  author={Hsin-Yi Henry Ho and Rajat Rohatgi and Andres M Lebensohn and Marc W Kirschner},
  journal={Methods in enzymology},
  year={2006},
  volume={406},
  pages={174-90}
}
In the accompanying chapter, we describe an in vitro system that uses Xenopus egg extracts to study actin assembly induced by phosphatidylinositol (4,5)bisphosphate (PIP2) and Cdc42. Biochemical fractionation and candidate screening experiments conducted in the extract system have identified the Arp2/3 complex, the N-WASP-WIP (or N-WASP-CR16) complex, and the Cdc42-binding protein Toca-1 as important mediators of PIP2- and Cdc42-actin signaling. Toward our ultimate goal of reconstituting an in… CONTINUE READING

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