In vitro reconstitution of a single-stranded transposition mechanism of IS608.

@article{Guynet2008InVR,
  title={In vitro reconstitution of a single-stranded transposition mechanism of IS608.},
  author={Catherine Guynet and Alison Burgess Hickman and Orsolya Barabas and Fred Dyda and Michael Chandler and Bao Ton-Hoang},
  journal={Molecular cell},
  year={2008},
  volume={29 3},
  pages={302-12}
}
Bacterial insertion sequences (IS) play an important role in restructuring their host genomes. IS608, from Helicobacter pylori, belongs to a newly recognized and widespread IS group with a unique transposition mechanism. We have reconstituted the entire set of transposition cleavage and strand transfer reactions in vitro and find that, unlike any other known transposition system, they strictly require single-strand DNA. TnpA, the shortest identified transposase, uses a nucleophilic tyrosine for… CONTINUE READING