In vitro reconstitution of monogalactosyldiacylglycerol (MGDG) synthase regulation by thioredoxin.

@article{Yamaryo2006InVR,
  title={In vitro reconstitution of monogalactosyldiacylglycerol (MGDG) synthase regulation by thioredoxin.},
  author={Yoshiki Yamaryo and Ken Motohashi and Ken-ichiro Takamiya and Toru Hisabori and Hiroyuki Ohta},
  journal={FEBS letters},
  year={2006},
  volume={580 17},
  pages={
          4086-90
        }
}
Monogalactosyldiacylglycerol (MGDG), a major membrane lipid of chloroplasts, is synthesized by MGDG synthase (MGD) localized in chloroplast envelope membranes. We investigated whether MGD activity is regulated in a redox-dependent manner using recombinant cucumber MGD overexpressed in Escherichia coli. We found that MGD activity is reversibly regulated by reduction and oxidation in vitro and that an intramolecular disulfide bond(s) is involved in MGD activation. Because thioredoxin efficiently… CONTINUE READING

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