In vitro recombination cloning of entire cDNA libraries in Arabidopsis thaliana and its application to the yeast two-hybrid system

@article{Buerkle2005InVR,
  title={In vitro recombination cloning of entire cDNA libraries in Arabidopsis thaliana and its application to the yeast two-hybrid system},
  author={Lukas Buerkle and Svenja Meyer and Hakan Dortay and Hans Lehrach and Alexander Heyl},
  journal={Functional & Integrative Genomics},
  year={2005},
  volume={5},
  pages={175-183}
}
In the postgenomic era many experiments rely on the availability of transcript sequence for cloning. As these clones usually originate from cDNA libraries, the quality of these libraries is crucial. If a good library is generated it is desirable to use a versatile cloning system suitable for many different kinds of applications. The cloning systems based on in vitro recombination proves fitting for this task. However, the use of this method for shuttling entire cDNA libraries between different… CONTINUE READING

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