In vitro protein splicing of purified precursor and the identification of a branched intermediate.

@article{Xu1993InVP,
  title={In vitro protein splicing of purified precursor and the identification of a branched intermediate.},
  author={Ming-Qun Xu and Maurice W. Southworth and Fana B Mersha and L J Hornstra and Francine B. Perler},
  journal={Cell},
  year={1993},
  volume={75 7},
  pages={1371-7}
}
Protein splicing is a posttranslational processing event in which an internal polypeptide is excised from a protein precursor and the terminal polypeptides are then ligated together, resulting in the production of two proteins. This report presents direct evidence for protein splicing by demonstrating in vitro splicing of purified precursor that accumulated when the protein splicing element from Pyrococcus DNA polymerase was cloned into a foreign gene. In vitro splicing was temperature and pH… CONTINUE READING