In vitro propagation of primary and extended life span murine corneal endothelial cells.

@article{Joo1994InVP,
  title={In vitro propagation of primary and extended life span murine corneal endothelial cells.},
  author={Choun – Ki Joo and Jay S. Pepose and Timothy P. Fleming},
  journal={Investigative ophthalmology & visual science},
  year={1994},
  volume={35 11},
  pages={3952-7}
}
PURPOSE To establish primary and extended life span cell cultures of murine corneal endothelial cells for a model system investigating corneal endothelial cell replacement and the immunologic features of corneal graft rejection. METHODS The authors have been able to grow corneal endothelium in culture by isolating adult murine Descemet's membrane and allowing the endothelial cells to proliferate from the explants. To isolate extended life span murine corneal endothelial cells, cells were… CONTINUE READING
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