In vitro propagation of Stevia rebaudina plants using multiple shoot culture.

@article{Nepovm1998InVP,
  title={In vitro propagation of Stevia rebaudina plants using multiple shoot culture.},
  author={Ales Nepov{\'i}m and Tom{\'a}s Vanek},
  journal={Planta medica},
  year={1998},
  volume={64 8},
  pages={
          775-6
        }
}
A multiple shoot culture was induced from nodal segments on MS medium containing half concentration of macroelements, 1% sucrose, and supplemented with NAA (0.01 mg/l). A bioreactor with hormone-free MS medium (300 ml) was inoculated with 1.5 g of the multiple shoot culture and cultivated for a month. The cultivating process of the multiple shoot culture in the bioreactor and the transfer into ex vitro conditions took about 8-9 weeks and produced approx. 600 new seedlings, that could be… CONTINUE READING
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