In vitro propagation of Nosema locustae using fat body cell line derived from Mythimna convecta (Lepidoptera: Noctuidae).

Abstract

Nosema locustae, a microsporidian parasite of locusts and grasshoppers, was successfully propagated in a fat body cell line from Mythimna convecta (BPMNU-MyCo-1). The fat body cells were grown in MGM-448 medium supplemented with 5% fetal bovine serum and 3% Bombyx mori serum at 25 degrees C. Cultures were inoculated with Nosema spores and agitated for 2 min… (More)

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@article{Khurad1991InVP, title={In vitro propagation of Nosema locustae using fat body cell line derived from Mythimna convecta (Lepidoptera: Noctuidae).}, author={Arun M. Khurad and Susheel Kumar Raina and T N Pandharipande}, journal={The Journal of protozoology}, year={1991}, volume={38 6}, pages={91S-93S} }