In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.

@article{Montpetit2004InVP,
  title={In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.},
  author={Diane Montpetit and Maurice Lalonde},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2004},
  volume={15},
  pages={189-199}
}
Micropropagation of the actinorhizal plant Hippophae rhamnoides L. (sea-buckthorn) was achieved on Murashige and Skoog (MS) medium supplemented with 1 μM of benzylaminopurine (BA). A multiplication frequency of three to five shoots per explant was observed after 28 days. Rooting of these shoots was achieved in a medium containing 1/4 strength MS without growth regulators. The rooted plants were transferred to Turface R artificial substrate and inoculated with pure cultures of two Frankia… 

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High frequency shoot organogenesis and somatic embryogenesis in juvenile and adult tissues of seabuckthorn (Hippophae rhamnoides L.)

Seabuckthorn (Hippophae rhamnoides) is a multipurpose small tree with unique berries of high nutritional and pharmaceutical values and seedling offspring of this cultivar were investigated regarding induction of shoot organogenesis in leaf explants and in roots of intact seedlings, and induction of direct somatic embryogenesis in explants from shoot tissue.

Micropropagation of seabuckthorn (Hippophae rhamnoides ssp. turkestanica).

Comparison of multiple shoot development on different culture media revealed that there was increase in % multiple shooting, shoots/explant and callu s- ing along successive passages on WPM Medium, and there was decrease in overall explant survival from MS through ½MS to WPM, and decrease in explant vitrification from ½MS through MS to W PM.

Biotechnological interventions in sea buckthorn (Hippophae L.): current status and future prospects

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Product Development of Sea Buckthorn

The sea buckthorn industry has been thriving in Russia since the 1940s when scientists there began investigating the biologically active substances found in the berries, leaves, and bark.

Assessment of Genetic Stability of Propagated Plantlets of Four Sea Buckthorn (Hippophae) Cultivars and the Establishment of Genetic Relationships Between them by ISSR Markers

Information generated from this study can be used to select parents for hybrid development to maximize desirable agronomic traits in a breeding program aimed at developing segregating populations to map genes controlling special traits in H. rhamnoides.

Research and Biotechnology in Sea Buckthorn (Hippophae spp.)

Dried-shrink disease and drought are the main problems restricting the sustainable development of sea buckthorn, and molecular biotechnology may be able to provide for a solution to overcome these problems and limitations.

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