In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.

  title={In vitro propagation and subsequent nodulation of the actinorhizal Hippophae rhamnoides L.},
  author={Diane Montpetit and Maurice Lalonde},
  journal={Plant Cell, Tissue and Organ Culture},
Micropropagation of the actinorhizal plant Hippophae rhamnoides L. (sea-buckthorn) was achieved on Murashige and Skoog (MS) medium supplemented with 1 μM of benzylaminopurine (BA). A multiplication frequency of three to five shoots per explant was observed after 28 days. Rooting of these shoots was achieved in a medium containing 1/4 strength MS without growth regulators. The rooted plants were transferred to Turface R artificial substrate and inoculated with pure cultures of two Frankia… 

In vitro clonal multiplication of the actinorhizal plant Comptonia peregrina

Control light and aeration in liquid medium were critical conditions for successful micropropagation of the actinorhizal plant Comptonia peregrina.

Direct somatic embryogenesis from leaves, cotyledons and hypocotyls of Hippophae rhamnoides

Histological observation revealed that the somatic embryos were similar to those of zygotic embryos, and Granulated sugar was the optimal carbon source.

Micropropagation of sea buckthorn ( , HippophaerhaL . )

A protocol for micropropagation of sea buckthorn was developed starting with shoot tips or meristems from plants up to 18 years old. Among the different media used, the best medium for both


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High frequency shoot organogenesis and somatic embryogenesis in juvenile and adult tissues of seabuckthorn (Hippophae rhamnoides L.)

Seabuckthorn (Hippophae rhamnoides) is a multipurpose small tree with unique berries of high nutritional and pharmaceutical values and seedling offspring of this cultivar were investigated regarding induction of shoot organogenesis in leaf explants and in roots of intact seedlings, and induction of direct somatic embryogenesis in explants from shoot tissue.

Micropropagation of seabuckthorn (Hippophae rhamnoides ssp. turkestanica).

Comparison of multiple shoot development on different culture media revealed that there was increase in % multiple shooting, shoots/explant and callu s- ing along successive passages on WPM Medium, and there was decrease in overall explant survival from MS through ½MS to WPM, and decrease in explant vitrification from ½MS through MS to W PM.

Biotechnological interventions in sea buckthorn (Hippophae L.): current status and future prospects

Limited progress has been made for improvement of sea buckthorn through breeding programs due to long juvenile period and lack of QTL linkage map, which makes screening of mapping populations a time-consuming and labor-intensive task.

Product Development of Sea Buckthorn

The sea buckthorn industry has been thriving in Russia since the 1940s when scientists there began investigating the biologically active substances found in the berries, leaves, and bark.

Assessment of Genetic Stability of Propagated Plantlets of Four Sea Buckthorn (Hippophae) Cultivars and the Establishment of Genetic Relationships Between them by ISSR Markers

Information generated from this study can be used to select parents for hybrid development to maximize desirable agronomic traits in a breeding program aimed at developing segregating populations to map genes controlling special traits in H. rhamnoides.

Research and Biotechnology in Sea Buckthorn (Hippophae spp.)

Dried-shrink disease and drought are the main problems restricting the sustainable development of sea buckthorn, and molecular biotechnology may be able to provide for a solution to overcome these problems and limitations.



In vitro propagation and nodulation byFrankia of actinorhizal Russian Olive (Elaeagnus angustifolia L.)

It was ascertained that after in vitro propagation of Elaeagnus angustifolia, the plants retained their capacity to nodulate and sustain nitrogen fixation.

Requirements for in vitro propagation of seven nitrogen-fixing Alnus species

Studies on the in vitro propagation of Alnus crispa, A. glutinosa and A. rubra indicated interspecific as well as intraspecific variations in their requirements for in vitro culture, while the optimum type and concentration of sugar to be used in the multiplication medium varied with species.

The Effect of Activated Charcoal on Tissue Cultures: Adsorption of Metabolites Inhibiting Morphogenesis

It was shown by mass spectrometry that the media without charcoal contained high amounts of phenylacetic acid and p-OH-benzoic acid (Daucus, Allium, and Haplopappus), whereas the media with activated charcoal did not.

Effect of substrate nitrogen on the performance ofin vitro propagatedAlnus glutinosa clones inoculated with Sp+ and Sp−Frankia strains

The results support the recommendation that selection of symbionts according to performance should be carried out with an input of combined nitrogen, thus inreasing the efficiency of the Alnus-Frankia symbiosis.

Physiological and biochemical events leading to vitrification of plants cultured in vitro

It is hypothesized that vitrification results from a burst of ethylene controlled by the peroxidase-IAA-oxidases system, which would retroinhibit its own biosynthesis and as a consequence decrease the activities of PAL and acidic peroxIDases, thus hindering lignification processes.


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Taxonomy and distribution of non-legume nitrogen-fixing systems

In this chapter more attention will be paid to plants bearing Alnus-type nodules than to other non-legume nitrogen-fixing systems, partly because it has been with the former that the author’s main

Prospects for the Mass Production of Improved Stock of Forest Trees by Cell and Tissue Culture

An annotated chronological bibliography of cell and tissue culture studies with forest tree species is presented and a new prospect of adding traits and producing genetic combinations which could not be obtained by sexual crossing is presented.

Recipe for ferric salts of ethylenediaminetetraacetic Acid.

FeEDTA became the main source of iron for the greater part of water culture, thanks to Jacobson, and ferric salts of ethylenediaminetetraacetic acid soon met with general acceptance in water culture.

Potential Uses of Nitrogen-Fixing Trees and Shrubs in Commercial Forestry

The potential for commercial utilization of tree and shrub species that can fix molecular nitrogen symbiotically is examined. Recommendations are made for research to speed the practical use in