In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica

@article{Cheng2008InVP,
  title={In vitro production of metabolism-enhancing phytoecdysteroids from Ajuga turkestanica},
  author={Diana M. Cheng and Gad Gabra Yousef and Mary H. Grace and Randy B. Rogers and Jonathan Gorelick-Feldman and Ilya Raskin and Scott Ann Laster},
  journal={Plant Cell, Tissue and Organ Culture},
  year={2008},
  volume={93},
  pages={73-83}
}
In order to develop a sustainable source of metabolism-enhancing phytoecdysteroids, cell suspension and hairy root cultures were established from shoot cultures of wild-harvested Ajuga turkestanica, a medicinal plant indigenous to Uzbekistan. Precursors of phytoecdysteroids (acetate, mevalonic acid cholesterol) or methyl jasmonate (an elicitor) were added to subculture media to increase phytoecdysteroid accumulation. In cell suspension cultures, 20-hydroxyecdysone (20E) content increased 3- or… CONTINUE READING