In vitro production of cattle-water buffalo (Bos taurus - Bubalus bubalis) hybrid embryos

@article{Kochhar2002InVP,
  title={In vitro production of cattle-water buffalo (Bos taurus - Bubalus bubalis) hybrid embryos},
  author={Harpreet S Kochhar and K B Rao and Alberto Maria Luciano and Satish Mahadeorao Totey and Fulvio Gandolfi and Parvathi K. Basrur and William Allan King},
  journal={Zygote},
  year={2002},
  volume={10},
  pages={155 - 162}
}
Interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. In an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (Bos taurus) and water buffalo (Bubalus bubalis), cattle oocytes were exposed to buffalo sperm and buffalo oocytes were exposed to cattle sperm and the cleavage rate and the… Expand
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References

SHOWING 1-10 OF 37 REFERENCES
In vitro maturation, fertilization and development of follicular oocytes from buffalo (Bubalus bubalis).
TLDR
The results indicate that oocytes cultured in medium supplemented with BES and gonadotrophins reveal high rates of maturation and development to the blastocyst stage after fertilization with fresh ejaculated spermatozoa. Expand
In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis ): Effects of media, hormones and sera.
TLDR
Ham's F-10 + LH + FSH + E(2) supplemented with FCS was the most efficacious culture system of those studied for the in vitro maturation of buffalo oocytes, and the results indicate that the culture medium has a marked effect on the fertilization rate of buffalo Oocytes. Expand
Cleavage capability of water buffalo follicular oocytes classified by cumulus cells and fertilized in vitro.
TLDR
A substantial variation in fertilization and developmental rates (16.0% to 43.8%) was observed among 4 different bulls and late non-surgically into 14 buffalo recipients on day 6 or 7 of their estrous cycle. Expand
Differential cleavage and developmental rates and their correlation with cell numbers and sex ratios in buffalo embryos generated in vitro.
TLDR
There may be a sire effect on sex ratio of in vitro produced buffalo embryos, which is suggested to be better than that of slow-cleaving, slow-developing blastocysts as judged by cell numbers. Expand
Effects of bull and heparin and sperm concentrations on in vitro fertilization of buffalo (Bubalus bubalis ) oocytes matured in vitro.
TLDR
Analysis of sperm density revealed that a concentration of 2 x 10(6) spermatozoa yielded optimal fertilization rates in vitro, while higher sperm concentrations resulted in higher oocyte penetration rates but gave rise to polyspermy. Expand
Developmental failure of hybrid embryos originated after fertilization of bovine oocytes with ram spermatozoa
TLDR
The developmental ability of hybrid zygotes, produced by in vitro fertilization of in vitro matured bovine oocytes with ram sperm, was evaluated by gross morphology, autoradiographic detection of (5‐3H) uridine incorporation, and fine structure morphology and in hybrid embryos these parameters were remarkably lower and may reflect the developmental failure of hybrid embryos. Expand
Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants.
  • J. G. Thompson
  • Biology, Medicine
  • Reproduction, fertility, and development
  • 1997
TLDR
Differences in lambing and calving rates and the reports of abnormal fetal development from embryos produced in vitro are of major concern, as they suggest that the in vitro environment may affect subsequent developmental physiology. Expand
Embryo development in vitro to the blastocyst stage in cattle, pigs and sheep
TLDR
Success of embryo culture is high enough to warrant considerable optimism about further improvements in the efficiency of many embryo technologies, including nuclear transfer (cloning) and gene transfer. Expand
Characterization of developmental arrest in early bovine embryos cultured in vitro.
TLDR
The susceptibility of early bovine embryos to developmental arrest ("blocking") in vitro was examined and it is concluded that the block is a manifestation of in vitro exposure during the four- to eight-cell stage and the block, while irreversible, is not the result of overt embryonic death. Expand
The in vitro developmental competence of bovine oocytes can be related to the morphology of the ovary.
TLDR
It is concluded that ovarian morphology is a simple and noninvasive parameter for an effective selection of oocytes with better developmental competence. Expand
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