In vitro production of cattle-water buffalo (Bos taurus - Bubalus bubalis) hybrid embryos

  title={In vitro production of cattle-water buffalo (Bos taurus - Bubalus bubalis) hybrid embryos},
  author={Harpreet S Kochhar and K B Rao and Alberto Maria Luciano and Satish Mahadeorao Totey and Fulvio Gandolfi and Parvathi K. Basrur and William Allan King},
  pages={155 - 162}
Interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. In an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (Bos taurus) and water buffalo (Bubalus bubalis), cattle oocytes were exposed to buffalo sperm and buffalo oocytes were exposed to cattle sperm and the cleavage rate and the… 

Developmental failure of hybrid embryos generated by in vitro fertilization of water buffalo (Bubalus bubalis) oocyte with bovine spermatozoa

Differential staining showed that the blastomere stopped dividing after 8‐cell stage, and parameters clearly showed that there was developmental failure of hybrid embryos.

Advanced reproductive technology in the water buffalo.

  • M. Drost
  • Biology, Medicine
  • 2007

In vitro maturation of buffalo oocytes and fertilization by cattle spermatozoa.

It can be concluded that buffalo Oocytes might be fertilized using capacitated cattle spermatozoa and both 10% BFF and 5% BSA could be supplemented in maturation media to enhance the maturation rate as well as fertilization of buffalo oocytes.

Influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells.

It is demonstrated that interspecies/intergeneric mitochondrial injected bovine oocytes have the ability to develop to the blastocyst stage after parthenogenetic activation and that injected mtDNA was neither selectively destroyed nor enhanced through development.

A comparative pattern of lectin-binding in the endometrial glands of the uterus and placenta of healthy buffaloes and bovines at early gestation

The results found in this study suggest that although both species are closely related, glycosylation patterns in the placenta and uterus are different, thus providing a possible reason for embryo transfer not being possible between these species.

Identification of bovine and novel interferon‐τ alleles in the American plains bison (bison bison) by analysis of hybrid cattle x bison blastocysts

The feasibility of using hybrid embryos for the analysis of developmentally regulated gene expression in species where embryos may not be available is demonstrated, and the pattern of secretion of the embryonic signaling molecule interferon‐τ (IFN‐τ) is determined.

Effects of Exposure to Heavy Metals on Viability, Maturation, Fertilization, and Embryonic Development of Buffalo (Bubalus bubalis) Oocytes In Vitro

Cadmium and lead were found to have a dose-dependent effect on viability, morphological abnormities, maturation, cleavage and morula/blastocyst yield, and blastocyst hatching in buffalo oocytes.

Formation of nucleoli in interspecies nuclear transfer embryos derived from bovine, porcine, and rabbit oocytes and nuclear donor cells of various species.

The ability of bovine, porcine, and rabbit oocytes to activate embryonic nucleoli formation in the nuclei of somatic cells of different mammalian species is investigated and it is demonstrated that the time and cell stage of nucleoli Formation are under the control of recipient ooplasm.

Interspecies somatic cell nuclear transfer: advancements and problems.

There are controversial reports in the literature, but in most cases these experiments have underlined some of the cellular and molecular mechanisms that are incomplete during cell nucleus reprogramming, including the failure to organize nucleoli, silence somatic cell genes, activate the embryonic genome, and resume mitochondrial replication and function, thus indicating nucleus-cytoplasmic incompatibility.

Mitochondrial DNA transmission and confounding mitochondrial influences in cloned cattle and pigs

  • K. Takeda
  • Biology
    Reproductive medicine and biology
  • 2013
This review focuses on mitochondrial considerations influencing SCNT techniques in farm animals and indicates that donor somatic cell mitochondria have a role in the reconstructed cytoplasm.



In vitro maturation and fertilization of buffalo oocytes (Bubalus bubalis ): Effects of media, hormones and sera.

Cleavage capability of water buffalo follicular oocytes classified by cumulus cells and fertilized in vitro.

A substantial variation in fertilization and developmental rates (16.0% to 43.8%) was observed among 4 different bulls and late non-surgically into 14 buffalo recipients on day 6 or 7 of their estrous cycle.

Developmental failure of hybrid embryos originated after fertilization of bovine oocytes with ram spermatozoa

The developmental ability of hybrid zygotes, produced by in vitro fertilization of in vitro matured bovine oocytes with ram sperm, was evaluated by gross morphology, autoradiographic detection of (5‐3H) uridine incorporation, and fine structure morphology and in hybrid embryos these parameters were remarkably lower and may reflect the developmental failure of hybrid embryos.

Comparison between in vivo-derived and in vitro-produced pre-elongation embryos from domestic ruminants.

  • J. G. Thompson
  • Biology, Medicine
    Reproduction, fertility, and development
  • 1997
Differences in lambing and calving rates and the reports of abnormal fetal development from embryos produced in vitro are of major concern, as they suggest that the in vitro environment may affect subsequent developmental physiology.

Characterization of developmental arrest in early bovine embryos cultured in vitro.

The in vitro developmental competence of bovine oocytes can be related to the morphology of the ovary.

Interspecific pregnancy: barriers and prospects.

Investigations on the mechanisms that allow survival of the fetal allograft have been extended by pregnancies in which the fetus and pregnant female are from different species, and an immunological barrier appears to restrict interspecific pregnancy, but how the barrier is manifested appears to differ with species.