In vitro processing of recombinant G protein gamma subunits. Requirements for assembly of an active beta gamma complex.


The gamma subunits of the heterotrimeric G proteins (G gamma) are subject to carboxyl-terminal processing. This processing involves prenylation of a cysteine residue initially 4 amino acids from the carboxyl terminus, endoproteolytic truncation of the 3 terminal amino acids, and methylation of the now carboxyl-terminal prenylcysteine residue. The… (More)


  • Presentations referencing similar topics