In vitro priming with adenovirus/gp100 antigen-transduced dendritic cells reveals the epitope specificity of HLA-A*0201-restricted CD8+ T cells in patients with melanoma.

@article{Linette2000InVP,
  title={In vitro priming with adenovirus/gp100 antigen-transduced dendritic cells reveals the epitope specificity of HLA-A*0201-restricted CD8+ T cells in patients with melanoma.},
  author={Gerald P. Linette and Srinivas Shankara and Simonne Longerich and Sixun Yang and Rhonda Doll and Charles A Nicolette and Frederic I. Preffer and Bruce L. Roberts and Frank G. Haluska},
  journal={Journal of immunology},
  year={2000},
  volume={164 6},
  pages={3402-12}
}
Replication-deficient recombinant adenovirus (Ad) encoding human gp100 or MART-1 melanoma Ag was used to transduce human dendritic cells (DC) ex vivo as a model system for cancer vaccine therapy. A second generation E1/E4 region deleted Ad which harbors the CMV immediate-early promoter/enhancer and a unique E4-ORF6/pIX chimeric gene was employed as the backbone vector. We demonstrate that human monocyte-derived DC are permissive to Ad infection at multiplicity of infection between 100 and 500… CONTINUE READING

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