In vitro polymerization of tau protein monitored by laser light scattering: method and application to the study of FTDP-17 mutants.

@article{Gamblin2000InVP,
  title={In vitro polymerization of tau protein monitored by laser light scattering: method and application to the study of FTDP-17 mutants.},
  author={Truman C Gamblin and Martin E King and Hana N. Dawson and Michael P Vitek and Jeff Kuret and Robert W. Berry and Lester I. Binder},
  journal={Biochemistry},
  year={2000},
  volume={39 20},
  pages={6136-44}
}
Tau polymerization into the filaments that compose neurofibrillary tangles is seminal to the development of many neurodegenerative diseases. It is therefore important to understand the mechanisms involved in this process. However, a consensus method for monitoring tau polymerization in vitro has been lacking. Here we demonstrate that illuminating tau polymerization reactions with laser light and measuring the increased scattering at 90 degrees to the incident beam with a digital camera results… CONTINUE READING