In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.

Abstract

A visual assay to study megakaryocyte platelet release via proplatelet formation in vitro was established. Samples of megakaryocyte-enriched rat bone marrow were incubated (37 C) in RPMI-1640 medium with 15% autologous serum in specially prepared chambers. In the culture system, approximately 6% of megakaryocytes formed proplatelet processes within 24 hours. Inclusion of a heterologous antiplatelet antibody in the culture system inhibited proplatelet formation, compared with that in controls.

Cite this paper

@article{Handagama1987InVP, title={In vitro platelet release by rat megakaryocytes: effect of heterologous antiplatelet serum.}, author={Prem J. Handagama and Nemi C. Jain and Bernard F. Feldman and Thomas Buseck Farver and Constance S. Kono}, journal={American journal of veterinary research}, year={1987}, volume={48 7}, pages={1147-9} }