In vitro plant regeneration and de novo differentiation of secretory trichomes in Passiflora foetida L. (Passifloraceae)

  title={In vitro plant regeneration and de novo differentiation of secretory trichomes in Passiflora foetida L. (Passifloraceae)},
  author={Yara Brito Chaim Jardim Rosa and Marcelo Carnier Dornelas},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
The only species in the genus Passiflora (Passifloraceae) known to produce resin glands is P. foetida. These glands are secretory trichomes mainly present on the floral bracts and leaf stipules. The secretion produced by these glands has received attention recently due to the presence of substances with pharmacological properties. Attempts to apply in vitro cell culture methods for the large scale production of highly valuable metabolites has been rather limited due to the fact that these… 

In vitro organogenesis and efficient plant regeneration from root explants of Passiflora suberosa L. (Passifloraceae)

This report describes a highly efficient method for in vitro plant regeneration of P. suberosa obtained via organogenesis, using root segments as explants, and the highest number of regenerated plants per explant was obtained using Murashige and Skoog basal medium supplemented with 9 μM 6-benzyladenine.

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Flow cytometry analysis confirmed the genetic stability of the regenerants based on DNA quantity in comparison with seed-derived plantlets and established a protocol for in vitro organogenesis of Passiflora setacea and to determine the genetic Stability of regenerated plants.

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Progress in micropropagation of Passiflora spp. to produce medicinal plants: a mini-review

It seems that for awide range of applications of in vitro clones of Passiflora, interdisciplinary studies including genetic and phytochemical aspects are needed.

Species-dependent divergent responses to in vitro somatic embryo induction in Passiflora spp.

The results indicated that the in vitro conditions that promote somatic embryogenesis in some Passiflora species might induce shoot organogenesis in others, suggesting that the conservation of morphogenetic signals among Passif Gloria species might be limited by their phylogenetic relatedness.

Anatomical and ultrastructural analyses of in vitro organogenesis from root explants of commercial passion fruit (Passiflora edulis Sims)

This study provides important information for enhancing the understanding and characterization of the organogenic process in non-meristematic explants and provides information on the use of roots as explants in genetic transformation protocols for this important tropical species.

Direct Somatic Embryo Formation from Roots of In Vitro-Seedlings of Oil Palm (Elaeis Guineesis Jacq.)

Direct SE formation from roots is confirmed and the origin of SE is described, which was initiated from epidermal cells and a few parenchymatous cells and developed into SEs through somatic embryogenesis (SE).

Embryogenic potential of immature zygotic embryos of Passiflora: a new advance for in vitro propagation without plant growth regulators

Results demonstrate that IZE of P. miniata and P. speciosa possess sufficient levels of endogenous phytohormones to trigger a high rate of indirect somatic embryogenesis, and should be considered as explant for the establishment of somatic embryos in other species.

In vitro morphogenesis and secretion of secondary metabolites of Nicotiana tabacum tall glandular trichomes

The tall glandular trichomes occurring on young expanding leaves of Nicotiana tabacum grown in vitro were investigated using light and electron microscopy and histochemical and ultrastructural analysis of their secretory cells under in vitro conditions suggested their involvement in diterpene biosynthesis.



Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae)

Direct organogenesis in this species was obtained for the first time in this work, through shoot development from internodal segments in the presence of 6-benzyladenine (BA); the highest regeneration rates were achieved on MSM medium, regardless of the BA concentration.

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Somatic embryogenesis from zygotic embryos of Passiflora cincinnata Masters is induced, characterized by intense proliferation and maintenance of embryogenic competence after successive subcultures, and opens new prospects for massive propagation.

New insights into the in vitro organogenesis process: the case of Passiflora

It is shown that ultrastructural electron microscope findings are valuable ways to understand the in vitro regeneration process, in particular in the yellow passion fruit, and recommends the use of hypocotyls as a source of explant to obtain shoots as well as a genetic transformation system for theyellow passion fruit.

Age and Physiological Condition of Donor Plants Affect in vitro Morphogenesis in Leaf Explants of Passiflora edulis f. flavicarpa

In vitro shoot regeneration from leaf discs of Passiflora edulis f.

In vitro plant regeneration as a tool to improve ornamental characters in Passiflora species.

In vitro regenerated plants which regenerated from immature-flowers showed morphological alterations in the flower and in the fruit involucral bracts, conferring a new attractive phenotype.

Plant development and synthesis of essential oils in micropropagated and mycorrhiza inoculated plants of Origanum vulgare L. ssp. hirtum (Link) Ietswaart

Biomass production of micropropagated oregano was induced by inoculation with the fungus Glomus viscosum. The effects of arbuscular mycorrhizal (AM) symbiosis on morphological and metabolic


Histological analysis revealed the direct pathway of shoot regeneration through organogenesis, and surprisingly regenerants were recovered via both organogenesis and somatic embryogenesis for P. cincinnata.

Ploidy stability of somatic embryogenesis-derived Passiflora cincinnata Mast. plants as assessed by flow cytometry

In this study, flow cytometric analysis was used to evaluate the genetic stability of Passiflora cincinnata Mast. plants regenerated via primary and secondary somatic embryogenesis. Embryogenic calli

An efficient system for the production of clonal plantlets of the medicinally important aromatic plant: Salvia africana-lutea L.

The tissue culture of S. africana-lutea not only favoured rapid multiplication but was also characterized by seasonal in vitro flowering that was in synchrony with that of plants growing in the wild, making it highly attractive for implementation as an in vitro conservation strategy.

Identification, functional characterization and developmental regulation of sesquiterpene synthases from sunflower capitate glandular trichomes

Evidence for the transcriptional regulation of sesquiterpene synthase genes in trichome cells suggest a potential use for these specialized cells for the identification of further genes involved in the biosynthesis, transport, and regulation of S2C, particularly in sunflower trichomes.