In vitro oxygen-glucose deprivation to study ischemic cell death.

@article{Tasca2015InVO,
  title={In vitro oxygen-glucose deprivation to study ischemic cell death.},
  author={Carla In{\^e}s Tasca and Tharine A. Dal-Cim and Helena Cimarosti},
  journal={Methods in molecular biology},
  year={2015},
  volume={1254},
  pages={197-210}
}
Oxygen-glucose deprivation (OGD ) is widely used as an in vitro model for stroke, showing similarities with the in vivo models of brain ischemia. In order to perform OGD, cell or tissue cultures, such as primary neurons or organotypic slices, and acutely prepared tissue slices are usually incubated in a glucose-free medium under a deoxygenated atmosphere, for example in a hypoxic chamber. Here, we describe the step-by-step procedure to expose cultures and acute slices to OGD, focusing on the… CONTINUE READING