In vitro motility of immunoadsorbed brain myosin-V using a Limulus acrosomal process and optical tweezer-based assay.

@article{Wolenski1995InVM,
  title={In vitro motility of immunoadsorbed brain myosin-V using a Limulus acrosomal process and optical tweezer-based assay.},
  author={Joseph S. Wolenski and Richard E. Cheney and Mark S. Mooseker and Paul Forscher},
  journal={Journal of cell science},
  year={1995},
  volume={108 ( Pt 4)},
  pages={1489-96}
}
To facilitate functional studies of novel myosins, we have developed a strategy for characterizing the mechanochemical properties of motors isolated by immunoadsorption directly from small amounts of crude tissue extracts. In this initial study, silica beads coated with an antibody that specifically recognizes the tail of myosin-V were used to immunoadsorb this motor protein from brain extracts. The myosin-containing beads were then positioned with optical tweezers onto actin filaments… CONTINUE READING
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