In vitro modeling of nonhypoxic cold ischemia-reperfusion simulating lung transplantation.

@article{Casiraghi2009InVM,
  title={In vitro modeling of nonhypoxic cold ischemia-reperfusion simulating lung transplantation.},
  author={Monica Casiraghi and Jason R Tatreau and John B Abano and John W Blackwell and L. T. Watson and Keith Burridge and Scott H. Randell and Thomas M. Egan},
  journal={The Journal of thoracic and cardiovascular surgery},
  year={2009},
  volume={138 3},
  pages={760-7}
}
OBJECTIVE Although anoxia/reoxygenation of cultured cells has been used to model lung ischemia-reperfusion injury, this does not accurately mimic events experienced by lung cells while a lung is retrieved from a donor, stored, and transplanted. We developed an in vitro model of nonhypoxic ischemia-reperfusion injury to simulate these events. METHODS Human umbilical vein endothelial cells underwent simulated cold ischemia by replacing 37 degrees C culture media with 4 degrees C Perfadex… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-6 of 6 extracted citations

Cytoprotective and Antioxidant Effects of Steen Solution on Human Lung Spheroids and Human Endothelial Cells.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons • 2017
View 10 Excerpts
Highly Influenced

MAPK mediates inflammatory response and cell death in rat pulmonary microvascular endothelial cells in an ischemia-reperfusion model of lung transplantation.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation • 2013
View 1 Excerpt

Nitric oxide ventilation of rat lungs from non-heart-beating donors improves posttransplant function.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons • 2009
View 1 Excerpt

References

Publications referenced by this paper.
Showing 1-10 of 28 references

Impact of immediate primary lung allograft dysfunction on bronchiolitis obliterans syndrome.

American journal of respiratory and critical care medicine • 2007
View 2 Excerpts
Highly Influenced

Novel critical role of Toll-like receptor 4 in lung ischemia-reperfusion injury and edema.

American journal of physiology. Lung cellular and molecular physiology • 2009
View 8 Excerpts

Trends in lung pH and PO2 after circulatory arrest: implications for non-heart-beating donors and cell culture models of lung ischemia-reperfusion injury.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation • 2005
View 2 Excerpts

Biostatistics: a methodology for the health sciences

G van Belle, L Fisher, P Heagerty, T. Lumley
2004

Non-heart-beating donors.

Seminars in thoracic and cardiovascular surgery • 2004

Similar Papers

Loading similar papers…