In vitro mineralization of fetal rat parietal bones in defined serum-free medium: effect of beta-glycerol phosphate.

Abstract

We have developed a bone organ culture system that mineralizes in vitro. Fetal rat parietal bones (20 days old) were cultured in a chemically defined serum-free medium containing physiological 3 mM phosphate. During 5 days in culture, calcium content increased from 26 to 55 micrograms and dry weight increased from 137 to 194 micrograms. After 2 days in vivo… (More)

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@article{Gronowicz1989InVM, title={In vitro mineralization of fetal rat parietal bones in defined serum-free medium: effect of beta-glycerol phosphate.}, author={Gloria A. Gronowicz and Florence N. Woodiel and Maeve McCarthy and Lawrence G. Raisz}, journal={Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research}, year={1989}, volume={4 3}, pages={313-24} }