In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.

@article{Stein1982InVM,
  title={In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.},
  author={Roland Stein and Aharon Razin and Howard Cedar},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1982},
  volume={79 11},
  pages={3418-22}
}
The effect of DNA methylation on the expression of the hamster adenine phosphoribosyltransferase (aprt) gene in mouse cells has been examined. This gene was methylated in vitro at all of its C-C-G-G sites by using Hpa II methylase and was inserted into mouse Ltk- aprt- L cells by cotransformation, with the herpes virus thymidine kinase gene as a selectable… CONTINUE READING