In vitro metabolism of moxidectin in Haemonchus contortus adult stages

@article{Alvinerie2001InVM,
  title={In vitro metabolism of moxidectin in Haemonchus contortus adult stages},
  author={Michel Alvinerie and Jacques Dupuy and Claudine Eeckhoutte and J. F. Sutra and Dominique Kerboeuf},
  journal={Parasitology Research},
  year={2001},
  volume={87},
  pages={702-704}
}
We studied the implication of cytochrome P450 enzymes in the in vitro metabolism of moxidectin (MXD) in homogenates of Haemonchus contortus adult stages (susceptible isolate, Weybridge, UK). After homogenisation in a phosphate buffer, 2 ml of homogenates (equivalent to 1 g of nematodes) were incubated with 5 µg [14C] MXD at 37 °C for 24 h. MXD and its metabolites were separated by HPLC with radiodetection on-line. Only one metabolite was detected and its production was inhibited by carbon… CONTINUE READING